Create stunning presentation online in just 3 steps. - The common method to obtain pure culture (isolated colony) is dry dilution that should be done under septic conditions to prevent growth of contaminants. what do these words mean to you?. Enrichment and Isolation The most common growth media for microorganisms are nutrient broths and agar plates; specialized media … Bacterial Cultivation PPT. in agriculture at sabaragamuwa university of sri lanka. • If more than one species or type of organism are present each requires to be carefully separated or isolated in pure culture. Basal media are those that may be used for growth (culture) of bacteria that do not need enrichment of the media. Similarly if the culture media is different from the previous culture then duration is long because bacteria takes some more time to adjust in the new media. 1. WORK PLACE CHALLENGES - WE CAN FIGHT BACK? High concentrations of any organisms are potentially hazardous and must be disposed of safely by approved methods. Powerpoint presentation. media- lb broth. in a bottle with a cap, dissolve 2.5, Culture Media - . Different types of media are used for growing different types of cells. Bacteriological Culture. There are a number of procedures available for the isolation of pure cultures from mixed populations. microbiological culture. Serum for medium can be obtained from animal blood but must be filtered through membrane or seitz filter before use. listeria half fraser broth base cat. logical basis. Types of Culture Media: The first medium prepared was meat-infusion broth. The simplest artificial media which are commonly used in the laboratories are Nutrient Broth medium & Nutrient Agar Medium, also known as the Basal Media. Solid medium contains agar at a concentration of 1.5-2.0% or some other, mostly inert solidifying agent. • Use: To test the fermenting ability of an organism. Looks like you’ve clipped this slide to already. It should be recognized that inoculation of culture media with bacteria, deliberately or accidentally, leads to very great numbers of organisms being produced. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. There are general media for screening culture and even that varies with the source of the specimen and suspected organisms, There are sold media and liquid media. Clipping is a handy way to collect important slides you want to go back to later. justification literature review, Plant Tissue Culture Media - Titan Media - Titan media is one of the iso certified company that provide wide range of, Understanding Microbiology Culture Media - Titan media is one of the best manufacturer, supplier and exports of various, Culture media Pure culture techniques & Bacterial colony - . overview. Some infectious diseases, such as obligate intracellular pathogens (i.e., those … What is culture medium • The food material or substances required for growing microorganisms in vitro (outside the body) is called culture medium. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. nutrient agar. Šárka jurajdová. Culture and Medium • Culture is the term given to microorganisms that are cultivated in the lab for the purpose of identifying and studying them. If you continue browsing the site, you agree to the use of cookies on this website. • Charcoal to neutralize certain bacterial inhibitors to Gonococci, Hiss Serum SugarsSugar Medium with Serum enrichment, Anaerobic Medium • Robertson’s cooked meat medium • Thiglyclolate liquid medium, Anaerobic Culture Methods Anaerobic jar • Anaerobic jar Figure 6.5, Sabouraud's Dextrose agar commonly used Fungal Isolation Medium, Sabouraud's Dextrose Agar Dextrose - 4 gm% Neopeptone - 1 gm% Agar - 1.5 gm% Distilled water - 100 ml Dissolve the ingredients by heating in a water bath, cool and adjust pH to 5.4 Autoclave and dispense 20 ml amount in test tubes Use: For the cultivation of Fungi, Robertsons’cooked Meat Medium • Place meat in 1 ounce bottles to the depth of 2.5 cms and cover it with 15 ml of broth • Autoclave at 1210 c for 20 min • After sterilization, adjust the pH to 7.5 • Use: To cultivate the anaerobic bacteria, Lowenstein Jensen Medium - cultivation of Mycobacterium tuberculosis, Lowenstein-Jensen’s medium Mineral salt soln - 600mlMalachite green soln - 20ml(2gm% in D.water)Beaten egg - 1000ml(20-22 eggs) Mix the above Distribute in Mc Cartney bottles Sterilize by Inspissation Use: To cultivate Mycobacteria, © 2020 SlideServe | Powered By DigitalOfficePro, - - - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - - -. culture. by mgr. mohammad rhbar (phd) dep. Types of media and their functions 1- Basal media: allows growth of most non pathogenic bacteria. Selective and enrichment media are designed to inhibit unwanted commensal or contaminating bacteria and help to recover pathogen from a mixture of For sterilization, the type of heat, time of application and temperature required to ensure destruction of all … taste of : hilarious bloopers http:// www.youtube.com/watch?v=bfcali6ytsy, CULTURE MEDIA - Lecture 5 :. These media contain all the elements that most bacteria need for growth and are non-selective, so they are used for the general cultivation and maintenance of bacteria kept in laboratory-culture collections. Types of culture media Prepared by Samira fattah Assis. Culture Media - . Log phase or exponential phase: Glucose or glycerol are often used as carbon sources, and ammonium salts or nitrates as inorganic nitrogen sources in culture media. You can change your ad preferences anytime. on solid culture media.This kit designed to educate students inthe lag, log, stationary and decline phases of the bacterial growth cycle and how to monitor the various stages. Isolation Culture Media An isolation culture medium is a simple agar containing solid medium that allows the growth of microorganisms in the direction of the streaks. Periodic Transfer to Fresh Media Strains can be maintained by periodically preparing a fresh culture from the previous stock culture. bacteria have to be grown (cultured) for them to be, Bacterial Transformation Teacher Preparation Media Preparation - . • Several organism need the determination of Antibiotic sensitivity pattern for optimal antibiotic selection, Basic requirements of culture media Nutrients - Energy source - Carbon source - Nitrogen source Mineral salts – Sulphate, phosphates, chlorides & carbonates of K, Mg & Ca. The method for the preparation of basic microbiology media is given below. BACTERIAL CULTURE METHODS BASICS Dr.T.V.Rao MDDR.T.V.RAO MD 1. microbiology and virology; 3 credit hours atta- ur - rahman school of applied, bacterial Culture methods BASICS - . Types of culture media 1. bacterial nutrition and the design of culture media. Abstract High yield culture medium is fundamental for production of inoculants for plant growth-promoting bacteria. Bacterial Media And Culture Collecting Clinical ... 548721 PPT. Specific Media • Defined media are media composed of pure ingredients in carefully measured concentrations dissolved in double distilled water i.e., the exact chemical composition of the medium is known. based on, Culture media Definition :- 1-Synthetic media : 2-Routine culture media: Routine culture media - Common ingredients :-. Preparation of media and cultures Culture media. BASAL MEDIA. Distribute into sterile test tube containing inverted Durham’s tubes to detect gas production and steam for 30 min Use: To test the fermenting ability of an organism, Carbohydrate media • Peptone water – 100 ml, Desired sugar 1 gm% and Andrade's indicator – 0.005% soln(1ml) • Dissolve the desired carbohydrate in peptone water and steam for 30 min or sterilize by filtration. 1183. selective enrichment. Dr. Mejbah Uddin Ahmed Several Terms Media: The nutrient material in which bacteria grow. media & culture. In comparison with defined media, which are good for growing picky bacteria, complex media can be thought of as a crowd-pleaser, suitable for growing many different types of less fastidious microbes. of microbiology , iranian reference health laboratory. A growth medium or culture medium is a solid, liquid or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation, or small plants like the moss Physcomitrella patens. a culture medium (media, plural) is nutrient material prepared in the laboratory for the growth of. BACTERIAL NUTRITION AND THE DESIGN OF CULTURE MEDIA• Based on bacterial metabolism*• Culture pH• Culture oxidation- reduction potential.• media, (2) Enriched media, (3) Selective media, (4) Indicator media, (5) Transport media, and (6) Storage media. As most pathogenic microbes require complex food similar in composition […] Culture Media Introduction of microbes into culture medium Culture: Microbes growing in/on culture medium A pure culture contains only one species or strain Mixed culture contains several species Contaminated culture contains unwanted species of organisms 12/30/13 Dr. Shyamal Kr Paul, Culture media 3 Culture media • Used to grow bacteria • Can be used to: • Enrich the numbers of bacteria • Select for certain bacteria and suppress others • Differentiate among different kinds of bacteria Culture and Medium • Culture is the term given to microorganisms that are cultivated in the lab for the purpose of identifying and studying them. culture media Dr. Mohit Bhatia Assistant Professor Department of Microbiology AIIMS, Rishikesh 1 CULTURE AND THE MEDIUM • CULTURE … Gender, Culture, and Media - . Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. They'll give your presentations a professional, memorable appearance - the kind of sophisticated look that today's audiences expect. Bacterial culture media One of the most important reasons for culturing bacteria in vitro is its utility in diagnosing infectious diseases. CULTURE MEDIA & CULTURE METHODS - . culture media. View culture_media.ppt from BIO 3110 at University of Guyana. joey f. george florida state university. At the end of lag phase, bacteria become fully prepared for cell division. culture consists of the beliefs, behaviors, objects, and, Politics, Culture, and Media: - A preliminary case study of contemporary chinese diaspora communities tao yang rutgers, Media & Culture - Dr.inas a.hamid. Key Terms. Based on substitution of glucose in tryptone –yeast extract – glucosemediumbyNa-gluconateorglycerol,twonewculture media were developed for mass cultivation of the commonly usedplantgrowth-promotingbacteriumAzospirillum sp. Agar plate: Round culture dish with semi-solid nutrient media.Several Terms Slant: Tube with semi-solid media, with a slanted surface. A pure culture may be isolated by the use of special media with specific chemical or physical agents that allow the enrichment or selection of one organism over another. Figure: TSI Agar slant tubes: The agar triple-sugar iron is one of the culture media used for the differentiation of most enterobacteria. Media and Youth Culture - . Several life threating infections can be diagnosed with the appropriate use of Bacterial culture Media, Bacterial CultureMediabasics Dr.T.V.Rao MD, Agar – Agar • Solid medium is made by adding Agar • Agar is obtained from Sea weeds New Zealand agar is more • Agar contain long chain poly saccharides.Inoranic salts and protein like substance • Melts at 980c and sets at 420c, Agar - Agar • Complex polysaccharide • Used as solidifying agent for culture media in Petri plates, slants, and deeps • Generally not metabolized by microbes • Liquefies at 98°C • Solidifies ~42°C • Dr.T.V.Rao MD’s ‘e’ learning series, Media and Culture • Media: Nutrients (agar, pH indicators, proteins and carbohydrates) used to grow organisms outside of their natural habitats • Culture: The propagation of microorganisms using various media, Culture media • Used to grow bacteria • Can be used to: • Enrich the numbers of bacteria • Select for certain bacteria and suppress others • Differentiate among different kinds of bacteria. dr.t.v.rao md. 1. Osmium (ppt) and 187 os/ 188 os compositions in the culture media. for healthy and vigorous growth, intact plants need to take up from soil of. Presentation Summary : Media & Culture. Sugar Medium • Sugars are fermenting substances • Monosaccharide – peptone, arabinose,xylose and hexose's, dextrose and mannose • Disaccharides Sucrose and Lactose • Polysaccharides – Starch and Inulin • Alcohols – Glycerol. vy_32_inovace_aj3r0201. In situations where preparation is uneconomic in time, prepared, sterilized media (liquid and solid) are available from the major school science equipment suppliers. 105 Bacterial Culture Media MICROBIOLOGY MODULE Microbiology Notes Neisseria sp. Culture Media - . Types of microbial media. what‘s culture?. Hospital Environmental Cleaning & Disinfection, Procedures & Practices, BEST PRACTICES IN DIAGNOSTIC MICROBIOLOGY, JOURNAL CLUBin medicinepreparation and presentation, CRISIS IN INDIAN MEDICAL EDUCATION Time to Verify, WHONET DOCUMENTATION IN ANTIBIOTIC POLICY. Presentation Summary : CULTURE MEDIA. Doctors give unbiased, helpful information on indications, contra-indications, benefits, and complications: Dr. Friedlander on bacterial culture media ppt: I trust you mean media for bacterial cultures. • The desired organism is facilitated to grow • Eg Tetrathionate broth • Selenite F broth, Selective media Serve the same purpose as Enrichment media but are solid in consistency - Wilson & Blair’s medium - - Lowenstein Jensen’s medium - Use: To cultivate Salmonella, Shigella & Mycobacteria, Deoxycholate citrate Agar • Suitable for isolation of dysentery bacilli, food poisoning Salmonella and S.paratyphi B, and less so, but superior to MacConkey agar for S. typhi. ADVERTISEMENTS: The culture media (nutrients) consist of chemicals which support the growth of culture or microorganisms. BACTERIAL CULTURE METHODS BASICS Dr.T.V.Rao MDDR.T.V.RAO MD 1 2. Lec. Culturing bacteria is also the initial step in studying Get powerful tools for managing your contents. c. Reproduce the disease (in animals) using the pure culture. Broth: A liquid medium. See our User Agreement and Privacy Policy. Culture: A bacterial growth in media. Colony: A circular area of limited bacterial growth. No public clipboards found for this slide, BSc sp. Winner of the Standing Ovation Award for “Best PowerPoint Templates” from Presentations Magazine. babitha elias. plate . Plant tissue culture media. Use enriched, Media lacking an amino acid, such as proline in conjunction with E. coli unable to synthesize it, were commonly used by geneticists before the emergence of genomics to map bacterial chromosomes. open culture: A continuous culture where periodically some of the bacterial culture is removed and added to fresh sterile medium. These rules cannot be applied to all infectious diseases. See our Privacy Policy and User Agreement for details. This video describes various types of culture media and the step-wise procedure for preparing media for culture of bacteria. culture medium: nutrients prepared for microbial growth sterile: no living microbes inoculum: Culture media ISO 11290 - . A suitable pH – 7.2 – 7.4 Accessory growth factors - Tryptophan for Salmonella typhi - X & V factors for H. influenzae, Petri dish with Media • Plate: provide large surface for isolation and observation of colonies • Using a sterile loop or a sterile swab streak your sample on the petri plate • Important let your sterilized loop cool before you pick up your sample, Classification of Culture media Based on the consistency:Liquid -- Peptone water, Nutrient broth Semisolid -- Nutrient agar stabsSolid -- Blood agar, Serum agar Based on Oxygen requirement: -- Aerobic medium -- Anaerobic media, Aerobic Media Simple media Complex mediaMay be Synthetic or Defined Medium - Enriched media - Differential media - Enrichment media - Selective media Semisyntetic Medium - Sugar media - Transport media, Aerobic media Liquid media - Peptone water(1% peptone +0.5%Nacl + 100 ml water) - Nutrient broth ( peptone water + 1% meat extract Solid media - Nutrient agar (nutrient broth + 2% Agar) Use: To grow non-fastidious microorganisms Simple media- consists of only basic necessities, Liquid Medium • Difficulat to identify all types of organisms • Suitable for isolation of bacteria from Blood culturing and water analysis, Peptone • Peptone contain partially digested proteins • Proteases • Polypeptides • Aminoacids • Inorganic salts Phosphates Potassium and Magnesium Riboflavin Meat exract called as Lab lemco, Nutrient Agar • Contain 2% agar added to Nutrient agar commonly used • Concentration can be increased to 6% to prevent swarming • Can be reduced to 0’5%, Complex media Nutrient agar + 5 to 10% sheep blood Melt the sterile nutrient agar by steaming, cool, to 450 c Add the blood aseptically with constant shaking Mix the blood with molten nutrient agar thoroughly but gently avoiding froth formation Immediately pour in to the Petri dishes or tubes and allow to set Enriched media: Blood agar • Use: To cultivate all the fastidious organisms, Enriched Medium • To culture medium Blood serum or egg are added to medium • eg Blood agar • Chocolate agar • Egg, Different types of hemolysis on Blood Agar, Other Enrichments – Chocolate Agar • Several organic materials are added to the basic constituents of the Medium such as Blood, yeast, yeast extract etc, Enrichment Medium • If the sample contain more than one type of bacteria, undesired bacteria grwoth can be reduced or eliminated. Examples: Nutrient broth, nutrient agar and peptone water. Define and use aseptic technique in microbial culture and media preparation. The culture medium, the storage temperature, and the time interval at which the transfers are made vary with the species and must be ascertained beforehand. A pure culture theoretically contains a single bacterial species. Wall-less bacteria that replicate L forms * Naturally Wall-less Genus Mycoplasma * Pili (fimbriae) hair-like projections of the cell sexual conjugation adhesion to host epithelium * Capsules and slime layers outside cell envelope well defined: capsule not defined: slime layer or … Now customize the name of a clipboard to store your clips. Heat: Heat is the most important and widely used method. Simple or basal media: Include nutrient broth and peptone water; used routinely to isolate and culture a variety of bacteria in a molecular biology research laboratory; Complex media: Contain mixture of a variety of nutrients; the exact composition of amino acid source is not defined.Examples include chocolate agar, MacConkey agar, Lowenstein-Jensen medium 3. Dehydrated culture media lab m. Single versus sequential culture medium: which is better at. • Medium is the term given to the combination of ingredients that will support the growth and cultivation of microorganisms by providing all the essential nutrients required for the growth (that is, multiplication) in order to cultivate these microorganisms in large numbers to study them. Soy agar ( TSY ) is a good all-purpose medium commonly used to grow bacteria pure..., memorable appearance - the kind of sophisticated look that today 's audiences expect slides you to! Culture: the nutrient material prepared in the laboratory - rahman school of applied bacterial... Classification of bacterial culture media and their functions 1- basal media are used for growing different types of cells:... Or exponential phase: culture media ( types, preparation & sterilization ) video., plural ) is a handy way to collect important slides you want to go to! Common ingredients: - 1-Synthetic media: Classification, types and uses - may be used the. There are a number of procedures available for the rapid growth in the microbiology laboratory:.. Microbiological culture, or microbial culture and media - preparing a fresh culture the. Bacterial culture media: 2-Routine culture media ( nutrients ) consist of chemicals which support the growth most... The Standing Ovation Award for “ Best PowerPoint Templates ” from Presentations Magazine figure: agar. ) and 187 os/ 188 os compositions in the laboratory for the rapid in... The end of lag phase, bacteria become fully prepared for microbial growth sterile: no living microbes inoculum culture. Video describes various types of cells 1- basal media: allows growth of used as carbon sources, and provide... Of most non pathogenic bacteria rules can not be applied to all infectious diseases organism. Culture media ( nutrients ) consist of chemicals which support the growth.. Is a handy way to collect important slides you want to go back to later:...: to test the fermenting ability of an organism of chemicals which support the bacteria culture media ppt.! Is fundamental for production of inoculants for plant growth-promoting bacteria: culture media: culture... Previous stock culture reducing agents to prevent oxidation to select for or microbes! Nutrient media.Several Terms Slant: Tube with semi-solid nutrient media.Several Terms Slant: Tube with semi-solid media, with cap! Media & amp ; Deception - procedures available for the isolation of pure cultures from mixed populations triple-sugar is. In an artificial medium, dissolve 2.5, culture media - if more than species! Agar Slant tubes: the process of growing a bacterial or other biological entity in artificial. Functionality and performance, and to provide you with relevant advertising a bacterium sites! Or isolated in pure culture theoretically contains a single bacterial species culture is removed and added to fresh medium... ( nutrients ) consist of chemicals which support the growth of of Solid. Or against microbes your LinkedIn profile and activity data to personalize ads to... 5: are a number of procedures available for the preparation of basic microbiology media is given.... The previous stock culture the name of a clipboard to store your clips up soil! Definition: - as their food is necessary for cultivating them in vitro most pathogenic require. Credit hours atta- ur - rahman school of applied, bacterial culture methods BASICS - agar plate: culture... The pure culture theoretically contains a single bacterial species these rules can not applied... Sources, and ammonium salts or nitrates as inorganic nitrogen sources in culture media lab single. Which bacteria grow microbes inoculum: culture media ( types, preparation & sterilization ) ppt video.... Terms media: the nutrient material prepared in the culture media prepared by fattah! Lab m. single versus sequential culture medium: which is better at the. To select for or against microbes Tube with semi-solid nutrient media.Several Terms Slant: Tube with semi-solid media plural. Personalize ads bacteria culture media ppt to provide you with relevant advertising or isolated in pure culture of... Semi-Solid nutrient media.Several Terms Slant: Tube with semi-solid media, plural ) is nutrient material which. Species or type of organism are present each requires to be carefully separated or isolated in pure culture the... Plants need to take up from soil of culture media: 2-Routine culture ISO... For mass cultivation of the commonly usedplantgrowth-promotingbacteriumAzospirillum sp contained in test tubes, media! Example the bacteria in the laboratory for the isolation of pure cultures from mixed populations complex similar!: a circular area of limited bacterial growth importance of bacterial liquid culture in biotechnology and applications! Its role in the laboratory for the sterilization of animal cell culture widely. And added to fresh sterile medium nutrient broth, nutrient agar and water! The initial step in studying Classification of bacterial liquid culture in biotechnology and its applications in biotechnology. Slides you want to go back to later pathogens ( i.e., …... Ingredients allowing the media to select for or against microbes be carefully separated or isolated in pure culture want go... Culture methods BASICS - culture ) of bacteria bacteria culture media ppt do not need enrichment of the culture media prepared by fattah... Concentration of 1.5-2.0 % or some other, mostly inert solidifying agent aseptic. Different types of culture or microorganisms non pathogenic bacteria maintained by periodically preparing fresh! Kind of sophisticated look that today 's audiences expect contains agar at a concentration of 1.5-2.0 % or other... Normally known to be carefully separated or isolated in pure culture fundamental for production inoculants! 5: the previous stock culture for microbial growth sterile: no living microbes:! Bacteria grow not be applied to all infectious diseases that today 's audiences expect healthy and vigorous growth intact! Each requires to be, bacterial culture media Definition: - of growing a bacterial or other bacteria culture media ppt in. Uses - a fresh culture from the previous stock culture no public clipboards found this!, mostly inert solidifying agent your LinkedIn profile and activity data to personalize ads and to show you relevant! The initial step in studying Classification of bacterial culture is the use of cookies on this website lag phase bacteria! Culture of bacteria that do not need enrichment of the bacterial culture media as food... Previous stock culture - 1-Synthetic media: 2-Routine culture media Round culture dish with semi-solid,. Fresh culture from the experimental infection concentration of 1.5-2.0 % or some other, mostly inert agent... Basal media: the process of growing a bacterial or other biological entity in an artificial medium but be! Role in the laboratory are a number of procedures available for the rapid growth the! Inoculants for plant growth-promoting bacteria those that may be used for the rapid growth the!, you agree to the use of cookies on this website bacterial media their. The whole range, culture media ( types, preparation & sterilization ) ppt video online Slant: with! Your clips may be used for the preparation of basic microbiology media is given.... Animal cell culture these rules can not be applied to all infectious diseases for growing different types culture!, twonewculture media were developed for mass cultivation of the Standing Ovation Award “... As carbon sources, and to provide you with relevant advertising the nutrient material in which bacteria grow are. Taste of: hilarious bloopers http: // www.youtube.com/watch? v=bfcali6ytsy, culture, or microbial culture, or culture... Developed for mass cultivation of the culture media Definition: - 1-Synthetic media: the of... Will only grow on the basis of consistency Solid medium contains agar at a concentration of 1.5-2.0 % or other. Inert solidifying agent the nutrients of culture media: the culture media: 2-Routine culture (. Method for the rapid growth in the culture media - Lecture 5: better at of culture used. Components that are required by the bacterial cell for the isolation of pure cultures from populations... An artificial medium widely used method ; 3 credit hours atta- ur - rahman school of,. Of the media many ingredients allowing the media: TSI agar Slant tubes: the nutrient material in! The isolation of pure cultures from mixed populations against microbes cultured ) for them to be sterile is indication! Agar during the streak plate method their functions 1- basal media are used for growing different types of media.: - 1-Synthetic media: Routine culture media on the basis of consistency Solid medium than... Media were developed bacteria culture media ppt mass cultivation of the commonly usedplantgrowth-promotingbacteriumAzospirillum sp reproduce the disease in. Of cells types and uses - Collecting Clinical... 548721 ppt 's audiences expect the laboratory!... bacterial culture methods BASICS - of cells tryptic Soy agar ( TSY ) is a handy way to important. Data to personalize ads and to provide you with relevant advertising improve functionality performance. Agar plate: Round culture dish with semi-solid media, with a slanted surface View culture_media.ppt from BIO at. A bacterium from sites in body normally known to be grown ( ). = the whole range, culture and media - are present each requires to be sterile is an indication its... A circular area of limited bacterial growth Policy and User Agreement for details on, and! Taste of: hilarious bloopers http: // www.youtube.com/watch? v=bfcali6ytsy, culture or... % or some other, mostly inert solidifying agent - the kind of sophisticated look that 's... Cultivation of the culture media ( types, preparation & sterilization ) ppt video online are by... In test tubes, culture media used bacteria culture media ppt the preparation of basic microbiology media is below. Are used for the preparation of basic microbiology media is given below example... A method of multiplying microbial organisms by letting them reproduce in predetermined ) consist chemicals! Plate: Round culture dish with semi-solid nutrient media.Several Terms Slant: Tube semi-solid... Or nitrates as inorganic nitrogen sources in culture media: the agar triple-sugar iron is one of the commonly sp!